cd14 microbeads miltenyi biotec (Miltenyi Biotec)
Structured Review
Cd14 Microbeads Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cd1c/CD1c+(BDCA-1)%2B+Dendritic+Cell+Isolation+Kit%2C+human/10__1016_slash_j__isci__2026__116736-639-174-176
Average 94 stars, based on 23 article reviews
Images
Related Articles
Isolation:Article Title: Toxoplasma gondii infection of decidual CD1c(+) dendritic cells enhances cytotoxicity of decidual natural killer cells. Article Snippet: There is crosstalk between decidual natural killer (dNK) cells and decidual dendritic cells (dDCs) that promotes tolerance of trophoblast cells carrying paternally derived antigens.. In the present study, we report that infection of CD1c dDCs with Toxoplasma gondii enhanced gamma interferon (IFN-γ) production by dNK cells in co-culture.. The enhancement of IFN-γ production was induced by cytokine IL-12 which increased obviously in co-culture of dDCs with dNK cells following T. gondii infection, and this enhancement largely abrogated when cells were cultured in the presence of an anti-IL12 antibody. Article Title: Guidelines for visualization and analysis of DC in tissues using multiparameter fluorescence microscopy imaging methods. Article Snippet: .. BDCA-1+ DC were isolated directly from blood collection bags using the Article Title: Saponin-based adjuvants enhance antigen cross-presentation in human CD11c + CD1c + CD5 - CD163 + conventional type 2 dendritic cells. Article Snippet: PBMCs were isolated from buffy coats or apheresis from healthy donors (Sanquin, Nijmegen, The Netherlands) using Lymphoprep (STEMCELL Technologies). .. Total CD141+ CLEC9A+ DCs (cDC1s) were MACS isolated from PBMCs using the human CD141 (BDCA- 3) MicroBead kit (Miltenyi Biotec), followed by isolation of total CD1c+ DCs (cDC2s) using the Article Title: Immunomodulatory capacity of the serotonin receptor 5-HT2B in a subset of human dendritic cells Article Snippet: .. Article Title: Microenvironmental and cell intrinsic factors governing human cDC2 differentiation and monocyte reprogramming. Article Snippet: Monocytes were isolated using the CD14 MicroBeads (Miltenyi). .. For CD1c+ DC culture, PBMCs of two donors were pooled and cells were isolated using the Article Title: Agonists of toll-like receptor 3 and methods of their use Article Snippet: Human PBMC and Myeloid DC Cultures: Peripheral blood mononuclear cells (PBMCs) from freshly drawn healthy volunteer blood (Research Blood Components, Brighton, Mass.) were isolated by Ficoll density gradient centrifugation method (Ficoll-Paque PLUS, GE Health Care). .. Article Title: PD-1 blunts the function of ovarian tumor-infiltrating dendritic cells by inactivating NF-κB Article Snippet: Tumor specimens were minced into <1 mm 3 pieces, isolated into single cells using gentleMACSTM tissue dissociator (Miltenyi, San Diego, CA), passed through a 40μm filter and processed by ficoll gradient as previously described ( 10 , 25 ). .. CD1c + cells used in in vitro assays were isolated from the samples using Selection:Article Title: Toxoplasma gondii infection of decidual CD1c(+) dendritic cells enhances cytotoxicity of decidual natural killer cells. Article Snippet: There is crosstalk between decidual natural killer (dNK) cells and decidual dendritic cells (dDCs) that promotes tolerance of trophoblast cells carrying paternally derived antigens.. In the present study, we report that infection of CD1c dDCs with Toxoplasma gondii enhanced gamma interferon (IFN-γ) production by dNK cells in co-culture.. The enhancement of IFN-γ production was induced by cytokine IL-12 which increased obviously in co-culture of dDCs with dNK cells following T. gondii infection, and this enhancement largely abrogated when cells were cultured in the presence of an anti-IL12 antibody. Article Title: Agonists of toll-like receptor 3 and methods of their use Article Snippet: Human PBMC and Myeloid DC Cultures: Peripheral blood mononuclear cells (PBMCs) from freshly drawn healthy volunteer blood (Research Blood Components, Brighton, Mass.) were isolated by Ficoll density gradient centrifugation method (Ficoll-Paque PLUS, GE Health Care). .. Magnetic Cell Separation:Article Title: Saponin-based adjuvants enhance antigen cross-presentation in human CD11c + CD1c + CD5 - CD163 + conventional type 2 dendritic cells. Article Snippet: PBMCs were isolated from buffy coats or apheresis from healthy donors (Sanquin, Nijmegen, The Netherlands) using Lymphoprep (STEMCELL Technologies). .. Total CD141+ CLEC9A+ DCs (cDC1s) were MACS isolated from PBMCs using the human CD141 (BDCA- 3) MicroBead kit (Miltenyi Biotec), followed by isolation of total CD1c+ DCs (cDC2s) using the Cell Isolation:Article Title: Saponin-based adjuvants enhance antigen cross-presentation in human CD11c + CD1c + CD5 - CD163 + conventional type 2 dendritic cells. Article Snippet: PBMCs were isolated from buffy coats or apheresis from healthy donors (Sanquin, Nijmegen, The Netherlands) using Lymphoprep (STEMCELL Technologies). .. Total CD141+ CLEC9A+ DCs (cDC1s) were MACS isolated from PBMCs using the human CD141 (BDCA- 3) MicroBead kit (Miltenyi Biotec), followed by isolation of total CD1c+ DCs (cDC2s) using the Article Title: Immunomodulatory capacity of the serotonin receptor 5-HT2B in a subset of human dendritic cells Article Snippet: .. Article Title: Microenvironmental and cell intrinsic factors governing human cDC2 differentiation and monocyte reprogramming. Article Snippet: Monocytes were isolated using the CD14 MicroBeads (Miltenyi). .. For CD1c+ DC culture, PBMCs of two donors were pooled and cells were isolated using the In Vitro:Article Title: PD-1 blunts the function of ovarian tumor-infiltrating dendritic cells by inactivating NF-κB Article Snippet: Tumor specimens were minced into <1 mm 3 pieces, isolated into single cells using gentleMACSTM tissue dissociator (Miltenyi, San Diego, CA), passed through a 40μm filter and processed by ficoll gradient as previously described ( 10 , 25 ). .. CD1c + cells used in in vitro assays were isolated from the samples using |

