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cd14 microbeads miltenyi biotec  (Miltenyi Biotec)


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    Miltenyi Biotec cd14 microbeads miltenyi biotec
    Cd14 Microbeads Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cd1c/CD1c+(BDCA-1)%2B+Dendritic+Cell+Isolation+Kit%2C+human/10__1016_slash_j__isci__2026__116736-639-174-176
    Average 94 stars, based on 23 article reviews
    cd14 microbeads miltenyi biotec - by Bioz Stars, 2026-10
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    Related Articles

    Isolation:

    Article Title: Toxoplasma gondii infection of decidual CD1c(+) dendritic cells enhances cytotoxicity of decidual natural killer cells.
    Article Snippet: There is crosstalk between decidual natural killer (dNK) cells and decidual dendritic cells (dDCs) that promotes tolerance of trophoblast cells carrying paternally derived antigens.. In the present study, we report that infection of CD1c dDCs with Toxoplasma gondii enhanced gamma interferon (IFN-γ) production by dNK cells in co-culture.. The enhancement of IFN-γ production was induced by cytokine IL-12 which increased obviously in co-culture of dDCs with dNK cells following T. gondii infection, and this enhancement largely abrogated when cells were cultured in the presence of an anti-IL12 antibody.

    Article Title: Guidelines for visualization and analysis of DC in tissues using multiparameter fluorescence microscopy imaging methods.
    Article Snippet: .. BDCA-1+ DC were isolated directly from blood collection bags using the human CD1c (BDCA-1)+ DC Isolation Kit (Miltenyi) 2. ..

    Article Title: Saponin-based adjuvants enhance antigen cross-presentation in human CD11c + CD1c + CD5 - CD163 + conventional type 2 dendritic cells.
    Article Snippet: PBMCs were isolated from buffy coats or apheresis from healthy donors (Sanquin, Nijmegen, The Netherlands) using Lymphoprep (STEMCELL Technologies). .. Total CD141+ CLEC9A+ DCs (cDC1s) were MACS isolated from PBMCs using the human CD141 (BDCA- 3) MicroBead kit (Miltenyi Biotec), followed by isolation of total CD1c+ DCs (cDC2s) using the human CD1c (BDCA- 1)+ dendritic cell isolation kit (Miltenyi Biotec). .. A purity check of the subsets after isolation was performed by additional FACS staining and measured flow cytometry (BD FACSCanto II).

    Article Title: Immunomodulatory capacity of the serotonin receptor 5-HT2B in a subset of human dendritic cells
    Article Snippet: .. Human CD1c (BDCA-1)+ dendritic cells were isolated using a Miltenyi Cell Isolation Kit in accordance with the manufacturer’s protocol (Miltenyi Biotech). .. Phenotyping of resting and activated moDCs was performed by flow cytometry using anti-CD80-FITC, anti-CD83-FITC, anti-CD86-PE, anti-CD209-PE, anti-CD1a-PE, anti-CD14-PE (Beckman Coulter, Hialeah, FL), anti-HLA-DR-FITC and isotype-matched control antibody (Ab) (BD Pharmingen).

    Article Title: Microenvironmental and cell intrinsic factors governing human cDC2 differentiation and monocyte reprogramming.
    Article Snippet: Monocytes were isolated using the CD14 MicroBeads (Miltenyi). .. For CD1c+ DC culture, PBMCs of two donors were pooled and cells were isolated using the human CD1c (BDCA-1)+ Dendritic Cell Isolation Kit (Miltenyi) according to manufacturer’s protocol. ..

    Article Title: Agonists of toll-like receptor 3 and methods of their use
    Article Snippet: Human PBMC and Myeloid DC Cultures: Peripheral blood mononuclear cells (PBMCs) from freshly drawn healthy volunteer blood (Research Blood Components, Brighton, Mass.) were isolated by Ficoll density gradient centrifugation method (Ficoll-Paque PLUS, GE Health Care). .. Human CD1c (BDCA-1)+ myeloid dendritic cells were isolated from PBMCs by two magnetic separation steps involving depletion of CD19+ B cells and positive selection of CD1c (BDCA-1)+ cells (Miltenyi Biotec, Auburn, Calif.) according to the manufacturer's instructions. .. The culture medium used for the assay consisted of RPMI 1640 medium supplemented with 1.5 mM glutamine, 1 mM sodium pyruvate, 0.1 mM non-essential amino acids, 50 μM 2-mercaptoethanol, 100 IU/ml penicillin-streptomycin mix and 10% heat-inactivated fetal bovine serum (Hyclone).

    Article Title: PD-1 blunts the function of ovarian tumor-infiltrating dendritic cells by inactivating NF-κB
    Article Snippet: Tumor specimens were minced into <1 mm 3 pieces, isolated into single cells using gentleMACSTM tissue dissociator (Miltenyi, San Diego, CA), passed through a 40μm filter and processed by ficoll gradient as previously described ( 10 , 25 ). .. CD1c + cells used in in vitro assays were isolated from the samples using human CD1c + (BDCA-1 + ) DC isolation kit (Miltenyi). .. Blockade of human PD-1 was accomplished using a purified PD-1 antibody from BioLegend (Cat. # 329912, San Diego, CA).

    Selection:

    Article Title: Toxoplasma gondii infection of decidual CD1c(+) dendritic cells enhances cytotoxicity of decidual natural killer cells.
    Article Snippet: There is crosstalk between decidual natural killer (dNK) cells and decidual dendritic cells (dDCs) that promotes tolerance of trophoblast cells carrying paternally derived antigens.. In the present study, we report that infection of CD1c dDCs with Toxoplasma gondii enhanced gamma interferon (IFN-γ) production by dNK cells in co-culture.. The enhancement of IFN-γ production was induced by cytokine IL-12 which increased obviously in co-culture of dDCs with dNK cells following T. gondii infection, and this enhancement largely abrogated when cells were cultured in the presence of an anti-IL12 antibody.

    Article Title: Agonists of toll-like receptor 3 and methods of their use
    Article Snippet: Human PBMC and Myeloid DC Cultures: Peripheral blood mononuclear cells (PBMCs) from freshly drawn healthy volunteer blood (Research Blood Components, Brighton, Mass.) were isolated by Ficoll density gradient centrifugation method (Ficoll-Paque PLUS, GE Health Care). .. Human CD1c (BDCA-1)+ myeloid dendritic cells were isolated from PBMCs by two magnetic separation steps involving depletion of CD19+ B cells and positive selection of CD1c (BDCA-1)+ cells (Miltenyi Biotec, Auburn, Calif.) according to the manufacturer's instructions. .. The culture medium used for the assay consisted of RPMI 1640 medium supplemented with 1.5 mM glutamine, 1 mM sodium pyruvate, 0.1 mM non-essential amino acids, 50 μM 2-mercaptoethanol, 100 IU/ml penicillin-streptomycin mix and 10% heat-inactivated fetal bovine serum (Hyclone).

    Magnetic Cell Separation:

    Article Title: Saponin-based adjuvants enhance antigen cross-presentation in human CD11c + CD1c + CD5 - CD163 + conventional type 2 dendritic cells.
    Article Snippet: PBMCs were isolated from buffy coats or apheresis from healthy donors (Sanquin, Nijmegen, The Netherlands) using Lymphoprep (STEMCELL Technologies). .. Total CD141+ CLEC9A+ DCs (cDC1s) were MACS isolated from PBMCs using the human CD141 (BDCA- 3) MicroBead kit (Miltenyi Biotec), followed by isolation of total CD1c+ DCs (cDC2s) using the human CD1c (BDCA- 1)+ dendritic cell isolation kit (Miltenyi Biotec). .. A purity check of the subsets after isolation was performed by additional FACS staining and measured flow cytometry (BD FACSCanto II).

    Cell Isolation:

    Article Title: Saponin-based adjuvants enhance antigen cross-presentation in human CD11c + CD1c + CD5 - CD163 + conventional type 2 dendritic cells.
    Article Snippet: PBMCs were isolated from buffy coats or apheresis from healthy donors (Sanquin, Nijmegen, The Netherlands) using Lymphoprep (STEMCELL Technologies). .. Total CD141+ CLEC9A+ DCs (cDC1s) were MACS isolated from PBMCs using the human CD141 (BDCA- 3) MicroBead kit (Miltenyi Biotec), followed by isolation of total CD1c+ DCs (cDC2s) using the human CD1c (BDCA- 1)+ dendritic cell isolation kit (Miltenyi Biotec). .. A purity check of the subsets after isolation was performed by additional FACS staining and measured flow cytometry (BD FACSCanto II).

    Article Title: Immunomodulatory capacity of the serotonin receptor 5-HT2B in a subset of human dendritic cells
    Article Snippet: .. Human CD1c (BDCA-1)+ dendritic cells were isolated using a Miltenyi Cell Isolation Kit in accordance with the manufacturer’s protocol (Miltenyi Biotech). .. Phenotyping of resting and activated moDCs was performed by flow cytometry using anti-CD80-FITC, anti-CD83-FITC, anti-CD86-PE, anti-CD209-PE, anti-CD1a-PE, anti-CD14-PE (Beckman Coulter, Hialeah, FL), anti-HLA-DR-FITC and isotype-matched control antibody (Ab) (BD Pharmingen).

    Article Title: Microenvironmental and cell intrinsic factors governing human cDC2 differentiation and monocyte reprogramming.
    Article Snippet: Monocytes were isolated using the CD14 MicroBeads (Miltenyi). .. For CD1c+ DC culture, PBMCs of two donors were pooled and cells were isolated using the human CD1c (BDCA-1)+ Dendritic Cell Isolation Kit (Miltenyi) according to manufacturer’s protocol. ..

    In Vitro:

    Article Title: PD-1 blunts the function of ovarian tumor-infiltrating dendritic cells by inactivating NF-κB
    Article Snippet: Tumor specimens were minced into <1 mm 3 pieces, isolated into single cells using gentleMACSTM tissue dissociator (Miltenyi, San Diego, CA), passed through a 40μm filter and processed by ficoll gradient as previously described ( 10 , 25 ). .. CD1c + cells used in in vitro assays were isolated from the samples using human CD1c + (BDCA-1 + ) DC isolation kit (Miltenyi). .. Blockade of human PD-1 was accomplished using a purified PD-1 antibody from BioLegend (Cat. # 329912, San Diego, CA).



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    Image Search Results


    Ex vivo-generated cDC1s and cDC2s acquire a matured state upon TLR challenge. Enriched cDC1s and cDC2s were overnight stimulated with a maturation cocktail containing R848 and Poly(IC), and the next day, their phenotype and cytokine/chemokine production were characterized. (A) Representative histograms showing the expression levels of CD83, CD40, and CCR7 upon maturation on DC2s (blue histograms) and cDC1s (red histograms). (B) Maturation marker expression on cDC2s (blue dots) and cDC1s (red dots) is displayed as either percentage of positive DCs (CD80, CD86, CD83, CD40, and CCR7) or relative geometric mean fluorescence intensity (gMFI) (HLA-DR). Relative gMFI expression was determined by normalizing to the average gMFI of untreated cDC1s or cDC2s. Scatter dot plots show the mean ± SEM with each data point representing an individual DC donor ( n ≥ 8). (C) Scatter dot plots show the mean ± SEM of the raw concentrations of the indicated cytokines and chemokines. Each data point represents an individual DC donor ( n ≥ 8). Statistical significance was calculated for paired data sets with a paired t-test or a Wilcoxon test, whereas for unpaired data sets significancy was calculated using a Mann–Whitney test. * P < 0.05; ** P < 0.01; *** P < 0.001.

    Journal: Oncoimmunology

    Article Title: Ex vivo-generated conventional dendritic cells type 1 and type 2 from blood progenitors induce potent antigen-specific T-cell immunity

    doi: 10.1080/2162402X.2026.2695692

    Figure Lengend Snippet: Ex vivo-generated cDC1s and cDC2s acquire a matured state upon TLR challenge. Enriched cDC1s and cDC2s were overnight stimulated with a maturation cocktail containing R848 and Poly(IC), and the next day, their phenotype and cytokine/chemokine production were characterized. (A) Representative histograms showing the expression levels of CD83, CD40, and CCR7 upon maturation on DC2s (blue histograms) and cDC1s (red histograms). (B) Maturation marker expression on cDC2s (blue dots) and cDC1s (red dots) is displayed as either percentage of positive DCs (CD80, CD86, CD83, CD40, and CCR7) or relative geometric mean fluorescence intensity (gMFI) (HLA-DR). Relative gMFI expression was determined by normalizing to the average gMFI of untreated cDC1s or cDC2s. Scatter dot plots show the mean ± SEM with each data point representing an individual DC donor ( n ≥ 8). (C) Scatter dot plots show the mean ± SEM of the raw concentrations of the indicated cytokines and chemokines. Each data point represents an individual DC donor ( n ≥ 8). Statistical significance was calculated for paired data sets with a paired t-test or a Wilcoxon test, whereas for unpaired data sets significancy was calculated using a Mann–Whitney test. * P < 0.05; ** P < 0.01; *** P < 0.001.

    Article Snippet: After 2 h of coculture at 37oC, cells were stained to identify DC2s (CD1c-FITC, 1:25, 130-113-301, Miltenyi Biotec) and cDC1s (CD141 PerCP-Cy5.5, 1:25, 344112, BioLegend) prior to analysis using a BD FACSLyricTM.

    Techniques: Ex Vivo, Generated, Expressing, Marker, Fluorescence, MANN-WHITNEY

    CD34-derived cDC1s and cDC2s can be generated from blood progenitors. CD34-positive cells isolated from blood were differentiated into cDC1s and cDC2s in a 17-day protocol in which progenitors initially expand and afterward undergo skewing towards cDC1 and cDC2 differentiation. (A) Schematic representation of the 17-day protocol for DC generation. (B) Bar graph showing the percentage of cDC1s and cDC2s ± SEM at the end of the culture on day 17 ( n = 10). (C) Representative dot plot showing the presence of cDC1s and cDC2s based on the expression of CD141/CLEC9A and CD1c, respectively, after gating on viable single cells. (D) Violin plot showing the absolute number of cDC1s and cDC2s obtained with the current protocol, depending on the initial amount of cultured CD34-positive cells. Each dot represents the value of expanded cDC1s and cDC2s obtained from one independent donor ( n = 12). (E) Bar graphs show the percentage of positive DCs ± SEM for the given marker ( n = 3). CD34-derived cDC1s and cDC2s were characterized by flow cytometry to determine their lineage marker expression.

    Journal: Oncoimmunology

    Article Title: Ex vivo-generated conventional dendritic cells type 1 and type 2 from blood progenitors induce potent antigen-specific T-cell immunity

    doi: 10.1080/2162402X.2026.2695692

    Figure Lengend Snippet: CD34-derived cDC1s and cDC2s can be generated from blood progenitors. CD34-positive cells isolated from blood were differentiated into cDC1s and cDC2s in a 17-day protocol in which progenitors initially expand and afterward undergo skewing towards cDC1 and cDC2 differentiation. (A) Schematic representation of the 17-day protocol for DC generation. (B) Bar graph showing the percentage of cDC1s and cDC2s ± SEM at the end of the culture on day 17 ( n = 10). (C) Representative dot plot showing the presence of cDC1s and cDC2s based on the expression of CD141/CLEC9A and CD1c, respectively, after gating on viable single cells. (D) Violin plot showing the absolute number of cDC1s and cDC2s obtained with the current protocol, depending on the initial amount of cultured CD34-positive cells. Each dot represents the value of expanded cDC1s and cDC2s obtained from one independent donor ( n = 12). (E) Bar graphs show the percentage of positive DCs ± SEM for the given marker ( n = 3). CD34-derived cDC1s and cDC2s were characterized by flow cytometry to determine their lineage marker expression.

    Article Snippet: Alternatively, DCs were enriched by MACS using CD141-Microbeads (130-090-512, Miltenyi Biotec) and CD1c Isolation kit (130-119-475, Miltenyi Biotec) sequentially (see supplementary figure 1C).

    Techniques: Derivative Assay, Generated, Isolation, Expressing, Cell Culture, Marker, Flow Cytometry